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KMID : 0613820100200010027
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Journal of Life Science
2010 Volume.20 No. 1 p.27 ~ p.32
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Protective Effects of Fucoidan against UVB-Induced Oxidative Stress in Human Skin Fibroblasts
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Ku Mi-Jung
Lee Myeong-Sook
Moon Hee-Jung
Lee Yong-Hwan
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Abstract
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Ultraviolet-B (UVB) radiation induces the formation of reactive oxygen species (ROS) and depletes stores of cellular antioxidants. Fucoidan, polysaccharides containing L-fucose and sulfate ester groups, are constituents of brown algae. In this study, the protective effects of fucoidan on UVB-induced oxidative stress in cultured human skin fibroblast HS68 cells were assessed. Pretreatment with fucoidan significantly reduced malondialdehyde (MDA) content in a dose-dependent manner. With fucoidan pretreatment at a dose of 100 ㎍/ml, the level of intracellular glutathione was increased by 21.5%, compared to UVB irradiation alone. Fucoidan significantly reduced UVB-induced ROS generation by 40.1% and 68.4% at 10 and 100 ㎍/ml, respectively, compared to UVB irradiation alone. The positive staining rates of senescence-associated β-galactosidase were reduced by 23.1% and 16.4% with 10 and 100 ㎍/ml of fucoidan, compared to UVB irradiation alone. Fucoidan may exert a photoprotective effect against UVB radiation-induced oxidative stress.
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KEYWORD
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Fucoidan, ultraviolet B, antioxidation
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